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Pyrococcus furiosus is an extremophilic species of Archaea. It is notable for having an optimum growth temperature of 100°C (a temperature which would destroy most living organisms), and for being one of the few organisms identified as possessing enzymes containing tungsten, an element rarely found in biological molecules.
[edit] PropertiesPyrococcus furiosus is noted for its rapid doubling time of 37 minutes under optimal conditions. It appears as mostly regular cocci of 0.8 µm to 1.5 µm diameter with monopolar polytrichous flagellation. It grows between 70 °C (158 °F) and 103 °C (217 °F), with an optimum temperature of 100 °C (212 °F), and between pH 5 and 9 (with an optimum at pH 7). It grows well on yeast extract, maltose, cellobiose, β-glucans, starch, and protein sources (tryptone, peptone, casein and meat extracts). Growth is very slow, or nonexistent, on amino acids, organic acids, alcohols, and most carbohydrates (including glucose, fructose, lactose and galactose). The ability to grow on polysaccharides (maltose, cellobiose, starch) but not on the monomeric sugars suggests that oligosaccharides with various degrees of polymerization may be imported into the cell, and only afterwards hydrolyzed to glucose. [edit] UsesThe enzymes of Pyrococcus furiosus are extremely thermostable. Consequently the DNA Polymerase from Pyrococcus furiosus (also known as Pfu DNA polymerase) can be used in the polymerase chain reaction (PCR) DNA amplification process. [edit] DiscoveryPyrococcus furiosus was originally isolated anaerobically from geothermally heated marine sediments with temperatures between 90 °C (194 °F) and 100 °C (212 °F) collected at the beach of Porto Levante, Vulcano Island, Italy. It was first described by Dr. Karl Stetter of the University of Regensburg in Germany, and a colleague, Dr. Gerhard Fiala. [edit] GenomeThe sequencing of the complete genome of Pyrococcus furiosus was completed in 2001 by scientists at the University of Maryland Biotechnology Institute. The Maryland team found that the genome had 1,908 kilobases, coding for some 2,065 proteins. [edit] The extremophile's scientific nameThe name Pyrococcus means "fireberry" in Greek, to refer to the extremophile's round shape and ability to grow in temperatures of around 100 degrees Celsius. The species name furiosus means 'rushing' in Latin, and refers to the extremophile's doubling time. [edit] References
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