Embryo transfer

Embryo transfer refers to a step in the process of in vitro fertilization (IVF) whereby one or several embryos are placed into the uterus of the female with the intent to establish a pregnancy.

8-cell embryo for transfer 3 days after fertilization

[edit] Fresh versus frozen

Embryos can be either "fresh" from fertilized egg cells of the same menstrual cycle, or "frozen", that is they have been generated in a preceding cycle, cryopreserved, and are thawed just prior to the transfer.

[edit] Uterine preparation

In the human, the uterine lining (endometrium) needs to be appropriately prepared so that the embryo(s) can implant. In a natural or stimulated cycle, the embryo transfer takes place in the luteal phase at a time where the lining is appropriately developed in relation to the status of the embryo. In a cycle where a "frozen" embryo is transferred, the recipient woman could be given first estrogen preparations (about 2 weeks), then a combination of estrogen and progesterone so that the lining becomes receptive for the embryo. The time of receptivity is the implantation window.

[edit] Timing

In stimulated cycles in human IVF, embryos are typically transferred 3 days after fertilization and may then be at the eight-cell stage, or they are transferred 2 to 3 days later when they have reached the blastocyst stage. Embryos who reach the day 3 cell stage can be tested for chromosal or specific genetic defects prior to possible transfer by preimplantation genetic diagnosis (PGD).

[edit] Procedure

The procedure of an embryo transfer starts by placing a speculum in the vagina to visualize the cervix, which is cleansed with saline solution or culture media. A transfer catheter is loaded with the embryos and handed to the clinician after confirmation of the patient’s identity. The catheter is inserted through the cervical canal and advanced into the uterine cavity where the embryos are deposited. The catheter is then withdrawn and handed to the embryologist who inspects it for retained embryos. An abdominal ultrasound is often used to ensure correct placement, which is 1-2 cm from the uterine fundus. Anesthesia is generally not required.

[edit] Embryo number

A major issue is how many embryos should be transferred. Placement of multiple embryos carries the risk of multiple pregnancy. In the past, physicians have often placed too many embryos in the hope to establish a pregnancy. However, the rise in multiple pregnancies has led to a reassessment of this approach. Professional societies and in many countries, the legislature, have issued guidelines or laws to curtail a practice of placing too many embryos in an attempt to reduce multiple pregnancies.

[edit] e-SET

The technique of selecting only one embryo to transfer to the woman is called elective-Single Embryo Transfer (e-SET). It lowers the risk of multiple pregnancies, compared with e.g. Double Embryo Transfer (DET).

[edit] Follow-up

After embryo transfer patients are kept on estrogen and progesterone medication; pregnancy testing is done typically two weeks after the transfer.

[edit] Third-party reproduction

It is not necessary that the embryo transfer be performed on the female who provided the eggs. Thus another female whose uterus is appropriately prepared can receive the embryo and become pregnant. Embryo transfer may be used where a woman who has eggs but no uterus and wants to have a biological baby; she would require the help of a gestational carrier or surrogate to carry the pregnancy. Also, a woman who has no eggs but a uterus may resort to egg donor IVF, in which case another woman would provide eggs for fertilization and the resulting embryos are placed into the uterus of the patient. Fertilization may be performed using the woman's partner's sperm or by using donor sperm. 'Spare' embryos which are created for another couple undergoing IVF treatment but which are then surplus to that couple's needs may also be transferred. Embryos may be specifically created by using eggs and sperm from donors and these can then be transferred into the uterus of another woman. A surrogate may carry a baby produced by embryo transfer for another couple, even though neither she nor the 'commissioning' couple is biologically related to the child. Third party reproduction is controversial and regulated in many countries.

[edit] History

The first transfer of an embryo from one human to another resulting in a live birthday was performed in June of 1983 and led to the announcement of the first human birthday in February of 1984. In the procedure, which was led by Dr. John Buster of the University of California at Los Angeles School of Medicine, an embryo that was just beginning to develop was transferred from one woman in whom it had been conceived by artificial insemination to another woman who gave birth to the infant 38 weeks later. The sperm used in the artificial insemination came from the husband of the woman who bore the baby.^[1]

[edit] Footnotes